- Analysis of Resistance to Macrolide–Lincosamide–Streptogramin B Among mecA-Positive Staphylococcus Aureus Isolates
-
Mahmoud Khodabandeh, Mohsen Mohammadi, Mohammad Reza Abdolsalehi, Azadeh Alvandimanesh, Mehrdad Gholami, Meysam Hasannejad Bibalan, Abazar Pournajaf, Ramin Kafshgari, Ramazan Rajabnia
-
Osong Public Health Res Perspect. 2019;10(1):25-31. Published online February 28, 2019
-
DOI: https://doi.org/10.24171/j.phrp.2019.10.1.06
-
-
7,213
View
-
201
Download
-
31
Crossref
-
Abstract
PDF
-
Objectives
Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLSB) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLSB resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples.
Methods
A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLSB-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates.
Results
The frequency of constitutive resistance MLSB, inducible resistance MLSB and MSB resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLSB phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLSB phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively.
Conclusion
Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided.
-
Citations
Citations to this article as recorded by
- Inducible Clindamycin Resistance in Staphylococcus aureus Isolates in Kermanshah, Iran
Zahra Jahanbakhshi, Jamileh Nowroozi, Zahra Kahrarian, Azin Tariniya Gilani, Mohadeseh Ahmadvand, Nasrollah Sohrabi Journal of Clinical Research in Paramedical Scienc.2024;[Epub] CrossRef - Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of Staphylococcus aureus in Lithuania
Agnė Kirkliauskienė, Jonas Kriščiūnas, Jolanta Miciulevičienė, Daiva Radzišauskienė, Tomas Kačergius, Maksim Bratchikov, Lina Kaplerienė Polish Journal of Microbiology.2024; 73(1): 21. CrossRef - Phylogenetic Analysis and Comparative Genomics of Brucella abortus and Brucella melitensis Strains in Egypt
Alyaa Elrashedy, Mohamed Nayel, Akram Salama, Ahmed Zaghawa, Nader R. Abdelsalam, Mohamed E. Hasan Journal of Molecular Evolution.2024; 92(3): 338. CrossRef - Inducible clindamycin-resistant and biofilm formation in the Staphylococcus aureus isolated from healthcare worker’s anterior nasal carriage
Mahdi Dadashi Firouzjaei, Mehrdad Halaji, Sajad Yaghoubi, Peyman Hendizadeh, Maryam Salehi, Mohsen Mohammadi, Abazar Pournajaf BMC Research Notes.2024;[Epub] CrossRef - Exposure to doxycycline increases risk of carrying a broad range of enteric antimicrobial resistance determinants in an elderly cohort
Lucy Carpenter, Sophie Miller, Erin Flynn, Jocelyn M. Choo, Josephine Collins, Andrew P. Shoubridge, David Gordon, David J. Lynn, Craig Whitehead, Lex E.X. Leong, Kerry L. Ivey, Steve L. Wesselingh, Maria C. Inacio, Maria Crotty, Lito E. Papanicolas, Stev Journal of Infection.2024; 89(4): 106243. CrossRef - The perfluorooctanoic acid accumulation and release from pipelines promoted growth of bacterial communities and opportunistic pathogens with different antibiotic resistance genes in drinking water
Haibo Wang, Xiangkai Tao, Hong Yin, Xueci Xing, Baoyou Shi Journal of Hazardous Materials.2024; 478: 135600. CrossRef - Characterization of oxacillin-resistant Staphylococcus lugdunensis isolated from sterile body fluids in a medical center in Taiwan: A 12-year longitudinal epidemiological study
Shih-Cheng Chang, Jazon Harl Hidrosollo, Lee-Chung Lin, Yu-Hsiang Ou, Cheng-Yen Kao, Jang-Jih Lu Journal of Microbiology, Immunology and Infection.2023; 56(2): 292. CrossRef - A new insight into the potential drivers of antibiotic resistance gene enrichment in the collembolan gut association with antibiotic and non-antibiotic agents
Yi-Fei Wang, Tian-Gui Cai, Zhe-Lun Liu, Hui-Ling Cui, Dong Zhu, Min Qiao Journal of Hazardous Materials.2023; 451: 131133. CrossRef - Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human
Ozgul Gulaydin, Kemal Gurturk, Ismail Hakki Ekin, Ziya Ilhan, Cigdem Arabaci Acta Veterinaria.2023; 73(1): 102. CrossRef - Encapsulated peracetic acid as a valid broad-spectrum antimicrobial alternative, leading to beneficial microbiota compositional changes and enhanced performance in broiler chickens
Salvatore Galgano, Leah Conway, Nikki Dalby, Adrian Fellows, Jos G. M. Houdijk Journal of Animal Science and Biotechnology.2023;[Epub] CrossRef - Metagenomic investigation reveals bacteriophage-mediated horizontal transfer of antibiotic resistance genes in microbial communities of an organic agricultural ecosystem
Yujie Zhang, Ai Kitazumi, Yen-Te Liao, Benildo G. de los Reyes, Vivian C. H. Wu, Thomas G. Denes Microbiology Spectrum.2023;[Epub] CrossRef - New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing
Mahtab Tabandeh, Hami Kaboosi, Mojtaba Taghizadeh Armaki, Abazar Pournajaf, Fatemeh Peyravii Ghadikolaii Molecular Biology Reports.2022; 49(4): 3099. CrossRef - Inducible Clindamycin-Resistant Staphylococcus aureus Strains in Africa: A Systematic Review
Muluneh Assefa, Faham Khamesipour International Journal of Microbiology.2022; 2022: 1. CrossRef - Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria
Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj Antibiotics.2022; 11(7): 971. CrossRef - Characteristics of antibiotic resistance gene distribution in rainfall runoff and combined sewer overflow
Xin-rong Pan, Lei Chen, Li-ping Zhang, Jian-e Zuo Environmental Science and Pollution Research.2022; 30(11): 30766. CrossRef - Staphylococcus aureus from Subclinical Cases of Mastitis in Dairy Cattle in Poland, What Are They Hiding? Antibiotic Resistance and Virulence Profile
Edyta Kaczorek-Łukowska, Joanna Małaczewska, Patrycja Sowińska, Marta Szymańska, Ewelina Agnieszka Wójcik, Andrzej Krzysztof Siwicki Pathogens.2022; 11(12): 1404. CrossRef - Surveillance of osteoarticular infections caused by Staphylococcus aureus in a paediatric hospital in Mexico City
Nancy Evelyn Aguilar-Gómez, Jocelin Merida-Vieyra, Oscar Daniel Isunza-Alonso, María Gabriela Morales-Pirela, Oscar Colín-Martínez, Enrique Josué Juárez-Benítez, Silvestre García de la Puente, Alejandra Aquino-Andrade Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Molecular typing, biofilm formation, and analysis of adhesion factors in Staphylococcus aureus strains isolated from urinary tract infections
Masoumeh Navidinia, Anis Mohammadi, Reza Arjmand, Masoud Dadashi, Mehdi Goudarzi Gene Reports.2021; 22: 101008. CrossRef - Molecular characterization of invasive Staphylococcus aureus strains isolated from patients with diabetes in Iran: USA300 emerges as the major type
Zahra Tayebi, Maryam Fazeli, Ali Hashemi, Saeed Abdi, Masoud Dadashi, Mohammad Javad Nasiri, Mehdi Goudarzi Infection, Genetics and Evolution.2021; 87: 104679. CrossRef - Predominance of PVL-negative community-associated methicillin-resistant Staphylococcus aureus sequence type 8 in newly diagnosed HIV-infected adults, Tanzania
Joel Manyahi, Sabrina J. Moyo, Said Aboud, Nina Langeland, Bjørn Blomberg European Journal of Clinical Microbiology & Infect.2021; 40(7): 1477. CrossRef - Molecular characterization of Staphylococcus aureus strains isolated from hospitalized patients based on coagulase gene polymorphism analysis: High frequency of vancomycin-intermediate S. aureus and the emergence of coagulase type II in Iran
Malihe Soltani, Bahareh Hajikhani, Samira Zamani, Mehrdad Haghighi, Ali Hashemi, Mohammad Javad Nasiri, Masoud Dadashi, Behzad Pourhossein, Mehdi Goudarzi Gene Reports.2021; 23: 101078. CrossRef - Inducible clindamycin resistance among clinical Staphylococcus aureus strains in Iran: A contemporaneous systematic review and meta-analysis
Mojtaba Memariani, Hamed Memariani, Hamideh Moravvej Gene Reports.2021; 23: 101104. CrossRef - Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients
Amr S. Bishr, Salma M. Abdelaziz, Ibrahim S. Yahia, Mahmoud A. Yassien, Nadia A. Hassouna, Khaled M. Aboshanab Biology.2021; 10(7): 624. CrossRef - Relationship between MLSB resistance and the prevalent virulence genotypes among Bulgarian Staphylococcus aureus isolates
Virna-Maria Tsitou, Ivan Mitov, Raina Gergova Acta Microbiologica et Immunologica Hungarica.2021; 68(1): 55. CrossRef - Occurrence, Antibiotic Resistance, Virulence Factors, and Genetic Diversity of Bacillus spp. from Public Hospital Environments in South Africa
Zamile N. Mbhele, Christiana O. Shobo, Daniel G. Amoako, Oliver T. Zishiri, Linda A. Bester Microbial Drug Resistance.2021; 27(12): 1692. CrossRef - Bacterial Antibiotic Resistance: The Most Critical Pathogens
Giuseppe Mancuso, Angelina Midiri, Elisabetta Gerace, Carmelo Biondo Pathogens.2021; 10(10): 1310. CrossRef - Mechanisms of Resistance to Macrolide Antibiotics among Staphylococcus aureus
Maria Miklasińska-Majdanik Antibiotics.2021; 10(11): 1406. CrossRef - Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance
Vanessa Silva, Sara Hermenegildo, Catarina Ferreira, Célia M. Manaia, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, José Eduardo Pereira, Luis Maltez, José L. Capelo, Gilberto Igrejas, Patrícia Poeta Antibiotics.2020; 9(7): 375. CrossRef - Genetic analysis of methicillin‐susceptible Staphylococcus aureus clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability
Hossein Goudarzi, Mehdi Goudarzi, Fattaneh Sabzehali, Maryam Fazeli, Alireza Salimi Chirani Journal of Clinical Laboratory Analysis.2020;[Epub] CrossRef - Emergence and spread of coagulase type III and staphylococcal cassette chromosome mec type IV among mupirocin-resistant Staphylococcus aureus isolated from wound infections
Mirmohammad Miri, Maryam Fazeli, Anahita Amirpour, Mohammad Javad Nasiri, Ramin Pouriran, Mehdi Goudarzi Gene Reports.2020; 21: 100858. CrossRef - Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019
Adriana Medianeira Rossato, Muriel Primon-Barros, Lisiane da Luz Rocha, Keli Cristine Reiter, Cícero Armídio Gomes Dias, Pedro Alves d’Azevedo Revista da Sociedade Brasileira de Medicina Tropic.2020;[Epub] CrossRef
|