Figure 1Sensitivity of conventional RT-PCR, amplified with specific primers for filoviruses. Determination of the detection limit of filoviral RNAs using RT-PCR and 10-fold serial dilutions of RNA transcripts. Input RNA dilutions are indicated above the lanes. BEBOV = Bundibugyo EBOV; EBOV = Ebola virus; Lane M = 1-kb DNA ladder; Lane NC = negative control; MARV = Marburg virus; MMARV = MARV-Musoke; REBOV = Reston EBOV; RMARV = Ravn virus; RT-PCR = reverse transcription-polymerase chain reaction; SEBOV = Sudan EBOV; TEBOV = Taï Forest EBOV; ZEBOV = Zaire EBOV.
Figure 2Specificity of conventional RT-PCR, amplified with specific primers for filoviruses. BEBOV = Bundibugyo EBOV; EBOV = Ebola virus; Lane B = primers for the Bundibugyo strain; Lane M = primers for the Musoke strain; Lane NC = negative control; Lane RE = primers for the Reston strain; Lane RM = primers for the Ravn strain; Lane S = primers for the Sudan strain; Lane T = primers for the Tai Forest strain; Lane Z = primers for the Zaire strain; MARV = Marburg virus; MMARV = MARV-Musoke; REBOV = Reston EBOV; RMARV = Ravn virus; RT-PCR = reverse transcription-polymerase chain reaction; SEBOV = Sudan EBOV; TEBOV = Taï Forest EBOV; ZEBOV = Zaire EBOV.
Table 1Primers for reverse transcription-polymerase chain reaction used in the study.
Virus |
Primer |
Sequence (5′→3′) |
Position |
Size (bp) |
BEBOV |
Forward |
GCAGAAATATGCTGAATCTCGTGAAC |
1062 |
418 |
Reverse |
ATCATCCTCGTCCTCAAGGTCAAAA |
1479 |
REBOV |
Forward |
CCAACAATATGCTGAGTCCAGAGAA |
1062 |
419 |
Reverse |
CATCCTCATGATCGTCAAGATCG |
1480 |
SEBOV |
Forward |
ACACGTGAGTTGGACAACCTT |
1078 |
402 |
Reverse |
GTCATCGTCGTCGTCCAAATTGAA |
1479 |
TEBOV |
Forward |
AATCTCGCGAGCTTGACCAT |
1076 |
404 |
Reverse |
CTCGTCACCATCTTCAAGGTCAAA |
1479 |
ZEBOV |
Forward |
CGAACTTGACCATCTTGGACTTG |
1083 |
399 |
Reverse |
TCCTCGTCGTCCTCGTCTAGAT |
1481 |
MMARV |
Forward |
AGGCGACATGAACATCAGGAAATT |
1012 |
398 |
Reverse |
TCGTCCTCATTCAGCAGTGCAAAT |
1409 |
RMARV |
Forward |
GCGACATGAACACCAGGAAATTC |
1014 |
412 |
Reverse |
ATTTTCAAGAGTATCCTCGTCTTCG |
1425 |